HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

646 – 660 of 27,274 results

646

Thermo Scientific™ HyPURITY™ C18 Columns, 3μm Particle Size

High quality, highly pure silica columns

Pricing & Availability
Specifications

Pore Size	190 Å
Particle Size	3 μm
Carbon Load	13%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Silica
Column Type	Reversed Phase
pH	1 to 9
Surface Area	200 m²/g
USP Type	L1

647

MilliporeSigma™ Purospher™ STAR RP-18 Endcapped Hibar™ HR UHPLC Columns, 2 micron

For ultra-fast applications, where resolution, sensitivity and sample throughput are crucial

Pricing & Availability
Specifications

Pore Size	120 Å
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Quantity	1/Pk.
Surface Area	330 m²/g
Max. Pressure	8700 psi (600 bar)
Column Format	Column
Particle Size	2 μm
Type	HR UHPLC Column
Carbon Load	17%
Packing Material	Silica
Diameter (Metric)	2.1 mm
pH	1.5 to 10.5
USP Type	L1

648

MilliporeSigma™ LiChroCART™ Columns/Purospher™ STAR NH₂ Sorbents HPLC Cartridges, 5μm

Designed for carbohydrate analysis with a typical mobile phase consisting of acetonitrile and water

Pricing & Availability
Specifications

Pore Size	120 Å
Particle Size	5 μm
Type	Cartridge
Stationary Phase	Amino (NH2)
Phase	Reversed Phase, Normal Phase, Ion Exchange, HILIC
Packing Material	Silica
Column Type	Mixed Mode
pH	2 to 7.5
Quantity	1/Pk.
Surface Area	330 m²/g
Max. Pressure	2175 psi (150 bar) to 2900 psi (200 bar)
USP Type	L8
Column Format	Cartridge

649

MilliporeSigma™ Supelco™ Ascentis™ C8 HPLC Column, 5 μm Particle Size

The Ascentis™ C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excels in highly aqueous mobile phases as well as high organic mobile phases.

Pricing & Availability
Specifications

Pore Size	100 Å
Particle Size	5 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 8
Technique	Fused-Core HPLC
Surface Area	450 m2/g
Product Line	Ascentis

650

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-Si HPLC Column, 3 μm Particle Size

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.

Pricing & Availability
Specifications

Pore Size	120 Å
Particle Size	3 μm
Stationary Phase	Silica
Phase	Reversed Phase
Matrix	Silica Phase/Silica Gel, Spherical Particle Platform
Length (Metric)	15 cm
Surface Area	170 m2/g
Product Line	SUPELCOSIL

651

MilliporeSigma™ Supelco™ Astec™ Diol HPLC Column, 5 μm Particle Size

Pricing & Availability

652

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å Biphenyl HPLC Column, 2 μm Particle Size

Ascentis™ Express 90 Å Biphenyl columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	2 μm
Stationary Phase	Biphenyl (Dimethylbiphenyl) Bonding
Phase	Reversed Phase
Matrix	Biphenyl (Dimethylbiphenyl) Bonding Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 9
Technique	Fused-Core HPLC
Surface Area	120 m2/g
Product Line	Ascentis

653

Thermo Scientific™ POROS™ HQ Strong Anion Exchange HPLC Columns

Thermo Scientific™ POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Store at room temperature.
Stationary Phase	Quaternized Polyethylene Imine
Material	PEEK
Product Line	POROS

654

TSKgel™ Protein A-5PW HPLC Column, 20 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Protein A-5PW is a 20 μm, 4.6 mm ID x 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection.

Pricing & Availability

655

Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Columns

Separate challenging peptide mapping samples with Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns.

Pricing & Availability
Specifications

Pore Size	100 Å
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
For Use With (Application)	Bottom-up Proteomics, LC-MS
Surface Area	450 m²/g
Max. Pressure	22,000 psi (1500 bar)
Column Format	Analytical Column, EASY-Spray™
Particle Size	2 μm
Carbon Load	15%
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric)	75 μm
pH	2 to 8
Product Line	Easy Spray
USP Type	L1

656

Thermo Scientific™ MAbPac™ HIC-10 HPLC Column

Separate monoclonal antibodies (mAbs) and related biologics with high resolution, excellent biocompatibility, and selectivity using the unique chemistry of this hydrophobic interaction column.

Pricing & Availability
Specifications

Pore Size	1000 Å
Particle Size	5 μm
Temperature	60°C
Phase	Hydrophobic Interaction
Column Type	Hydrophobic Interaction
pH	2 to 8
Quantity	Each
Green Features	FSC
Product Line	MAbPac

657

MilliporeSigma™ LiChroCART™ RP-18 HPLC Cartridge, 250-4

Functions in reverse phase with RP-18 stationary phase. MilliporeSigma™ LiChroCART™ RP-18 HPLC Cartridge, 250-4 comes with customized packing (please specify packing material) and is 250mmL x 4mmI.D.

Pricing & Availability

658

MilliporeSigma™ SS Hibar™ LiChrospher™ Silica RT Normal Phase HPLC Column, 5 μm, 4 mm x 250 mm

Column Format: Analytical Column; Carbon Load: 13%; Pore Size: 100 Å; Column Phase: Normal Phase

Pricing & Availability

659

MilliporeSigma™ Supelco™ Astec™ CYCLOBOND™ I 2000 RSP Chiral HPLC Column, 5 μm Particle Size

Astec™ CYCLOBOND™ I 2000 RSP chiral HPLC column is basically comprised of native and derivatized β and γ cyclodextrins. It has been used for numerous chiral separations via selectively trapping the organic molecules in the cyclodextrin cavity.